Features Interactions Isoform Disease Linear motifs Fingerprint Network All partners

PDE4D_HUMAN

Evidence for PS localization

GO
G2C
SynGO
SynaptomeDB
HPA (protein expression in neurons)

Functions

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [View more on UniProt]

Protein features

Binary interactions with known binding regions

To display all evidence describing the interaction with a partner, click on the protein name left to the bars.

Network

Download full PS network for entry.

Isoforms

Disease-causing germline mutations

Position Amino acid Mutation Disease Overlap with binding region
190 Ser Ala Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
225 Pro Thr Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
226 Phe Ser Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
587 Thr Pro Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
227 Ala Ser Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
228 Gln Glu Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
301 Ser Thr Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
304 Ala Val Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
329 Val Ala Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
590 Glu Ala Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
673 Gly Asp Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -
678 Ile Thr Acrodysostosis2,withorwithouthormoneresistance(ACRDYS2) -

Linear motifs

No annotated instance was found. To search for linear motifs, use the ELM prediction server.

Fingerprint

Molecular function

Term Name % Distance from the top of the tree Annotated in PDE4D_HUMAN
GO:0043169 cation binding 45.7143 4 yes
GO:0046872 metal ion binding 45.7143 5 yes
GO:0000166 nucleotide binding 40.0000 4 yes
GO:0017076 purine nucleotide binding 40.0000 5 yes
GO:0032555 purine ribonucleotide binding 40.0000 4 yes
GO:0043168 anion binding 40.0000 4 yes
GO:0030554 adenyl nucleotide binding 37.1429 6 yes
GO:0032559 adenyl ribonucleotide binding 37.1429 5 yes
GO:0035639 purine ribonucleoside triphosphate binding 31.4286 4 no
GO:0005524 ATP binding 28.5714 5 no
GO:0016301 kinase activity 25.7143 4 no
GO:0016773 phosphotransferase activity, alcohol group as acceptor 25.7143 4 no
GO:0019900 kinase binding 25.7143 4 no
GO:0019901 protein kinase binding 25.7143 5 no
GO:0001664 G protein-coupled receptor binding 17.1429 4 yes
GO:0042578 phosphoric ester hydrolase activity 17.1429 4 yes
GO:0051117 ATPase binding 11.4286 4 yes
GO:0004112 cyclic-nucleotide phosphodiesterase activity 8.5714 6 yes
GO:0004114 3,5-cyclic-nucleotide phosphodiesterase activity 8.5714 7 yes
GO:0004115 3,5-cyclic-AMP phosphodiesterase activity 8.5714 8 yes
GO:0005246 calcium channel regulator activity 8.5714 5 yes
GO:0008081 phosphoric diester hydrolase activity 8.5714 5 yes
GO:0030551 cyclic nucleotide binding 8.5714 5 yes
GO:0030552 cAMP binding 8.5714 5 yes
GO:0031690 adrenergic receptor binding 8.5714 5 yes
GO:0099106 ion channel regulator activity 8.5714 4 yes
GO:0031698 beta-2 adrenergic receptor binding 5.7143 6 yes

Biological process

Term Name % Distance from top the of the tree Annotated in PDE4D_HUMAN
GO:0010646 regulation of cell communication 82.8571 4 yes
GO:0009966 regulation of signal transduction 80.0000 4 yes
GO:0048522 positive regulation of cellular process 74.2857 4 yes
GO:0048523 negative regulation of cellular process 71.4286 4 yes
GO:1902531 regulation of intracellular signal transduction 71.4286 5 yes
GO:0031323 regulation of cellular metabolic process 68.5714 4 yes
GO:0060255 regulation of macromolecule metabolic process 68.5714 4 yes
GO:0009889 regulation of biosynthetic process 65.7143 4 yes
GO:0010556 regulation of macromolecule biosynthetic process 65.7143 5 yes
GO:0031326 regulation of cellular biosynthetic process 65.7143 5 yes
GO:0051128 regulation of cellular component organization 62.8571 4 no
GO:0009893 positive regulation of metabolic process 62.8571 4 yes
GO:0010468 regulation of gene expression 62.8571 6 yes
GO:0080090 regulation of primary metabolic process 62.8571 4 yes
GO:0031325 positive regulation of cellular metabolic process 60.0000 5 yes
GO:0048584 positive regulation of response to stimulus 60.0000 4 yes
GO:0051049 regulation of transport 57.1429 4 yes
GO:0010647 positive regulation of cell communication 54.2857 5 no
GO:0023056 positive regulation of signaling 54.2857 4 no
GO:0010604 positive regulation of macromolecule metabolic process 54.2857 5 yes
GO:0036211 protein modification process 51.4286 4 no
GO:0043412 macromolecule modification 51.4286 4 no
GO:0006796 phosphate-containing compound metabolic process 51.4286 4 yes
GO:0009891 positive regulation of biosynthetic process 51.4286 5 yes
GO:0031328 positive regulation of cellular biosynthetic process 51.4286 6 yes
GO:0009892 negative regulation of metabolic process 48.5714 4 yes
GO:0010557 positive regulation of macromolecule biosynthetic process 48.5714 6 yes
GO:0023057 negative regulation of signaling 48.5714 4 yes
GO:0031324 negative regulation of cellular metabolic process 48.5714 5 yes
GO:0048585 negative regulation of response to stimulus 48.5714 4 yes
GO:0051246 regulation of protein metabolic process 48.5714 5 yes
GO:0009967 positive regulation of signal transduction 45.7143 5 no
GO:0019219 regulation of nucleobase-containing compound metabolic process 45.7143 5 no
GO:0050804 modulation of chemical synaptic transmission 45.7143 5 no
GO:0099177 regulation of trans-synaptic signaling 45.7143 4 no
GO:0009968 negative regulation of signal transduction 45.7143 5 yes
GO:0010605 negative regulation of macromolecule metabolic process 45.7143 5 yes
GO:0010648 negative regulation of cell communication 45.7143 5 yes
GO:0051240 positive regulation of multicellular organismal process 45.7143 4 yes
GO:0071310 cellular response to organic substance 45.7143 4 yes
GO:0051130 positive regulation of cellular component organization 42.8571 5 no
GO:0019220 regulation of phosphate metabolic process 40.0000 6 yes
GO:0031399 regulation of protein modification process 40.0000 6 yes
GO:0051174 regulation of phosphorus metabolic process 40.0000 5 yes
GO:1901701 cellular response to oxygen-containing compound 40.0000 4 yes
GO:1902532 negative regulation of intracellular signal transduction 37.1429 6 yes
GO:0043067 regulation of programmed cell death 37.1429 4 no
GO:0043408 regulation of MAPK cascade 37.1429 6 no
GO:1902533 positive regulation of intracellular signal transduction 37.1429 6 no
GO:0006355 regulation of DNA-templated transcription 37.1429 7 no
GO:0051050 positive regulation of transport 37.1429 4 no
GO:0051252 regulation of RNA metabolic process 37.1429 5 no
GO:2001141 regulation of RNA biosynthetic process 37.1429 6 no
GO:0010628 positive regulation of gene expression 37.1429 7 yes
GO:0050776 regulation of immune response 37.1429 4 yes
GO:0006468 protein phosphorylation 34.2857 5 no
GO:0016310 phosphorylation 34.2857 5 no
GO:0042981 regulation of apoptotic process 34.2857 5 no
GO:0045595 regulation of cell differentiation 34.2857 4 no
GO:0080134 regulation of response to stress 34.2857 4 no
GO:0009890 negative regulation of biosynthetic process 34.2857 5 no
GO:0010558 negative regulation of macromolecule biosynthetic process 34.2857 6 no
GO:0031327 negative regulation of cellular biosynthetic process 34.2857 6 no
GO:0001932 regulation of protein phosphorylation 34.2857 7 yes
GO:0002684 positive regulation of immune system process 34.2857 4 yes
GO:0010959 regulation of metal ion transport 34.2857 6 yes
GO:0034762 regulation of transmembrane transport 34.2857 4 yes
GO:0042325 regulation of phosphorylation 34.2857 7 yes
GO:0043269 regulation of monoatomic ion transport 34.2857 5 yes
GO:0051248 negative regulation of protein metabolic process 34.2857 6 yes
GO:0051924 regulation of calcium ion transport 34.2857 7 yes
GO:0141124 intracellular signaling cassette 34.2857 4 yes
GO:1903169 regulation of calcium ion transmembrane transport 31.4286 7 yes
GO:1904062 regulation of monoatomic cation transmembrane transport 31.4286 6 yes
GO:0030334 regulation of cell migration 31.4286 5 no
GO:0042127 regulation of cell population proliferation 31.4286 4 no
GO:0051247 positive regulation of protein metabolic process 31.4286 6 no
GO:2000145 regulation of cell motility 31.4286 4 no
GO:0006357 regulation of transcription by RNA polymerase II 31.4286 8 no
GO:0006996 organelle organization 31.4286 4 no
GO:0008104 protein localization 31.4286 4 no
GO:0060341 regulation of cellular localization 31.4286 4 no
GO:0001817 regulation of cytokine production 31.4286 4 yes
GO:0010243 response to organonitrogen compound 31.4286 4 yes
GO:0034765 regulation of monoatomic ion transmembrane transport 31.4286 5 yes
GO:0044057 regulation of system process 31.4286 4 yes
GO:0051241 negative regulation of multicellular organismal process 31.4286 4 yes
GO:0007167 enzyme-linked receptor protein signaling pathway 28.5714 4 no
GO:0030030 cell projection organization 28.5714 4 no
GO:0030335 positive regulation of cell migration 28.5714 6 no
GO:0031344 regulation of cell projection organization 28.5714 5 no
GO:0031401 positive regulation of protein modification process 28.5714 7 no
GO:0040017 positive regulation of locomotion 28.5714 4 no
GO:0051094 positive regulation of developmental process 28.5714 4 no
GO:0060627 regulation of vesicle-mediated transport 28.5714 4 no
GO:0070372 regulation of ERK1 and ERK2 cascade 28.5714 7 no
GO:0071702 organic substance transport 28.5714 4 no
GO:0071705 nitrogen compound transport 28.5714 4 no
GO:0120035 regulation of plasma membrane bounded cell projection organization 28.5714 6 no
GO:2000147 positive regulation of cell motility 28.5714 5 no
GO:0022607 cellular component assembly 28.5714 4 no
GO:0033043 regulation of organelle organization 28.5714 5 no
GO:0044087 regulation of cellular component biogenesis 28.5714 4 no
GO:0010629 negative regulation of gene expression 28.5714 7 no
GO:0032880 regulation of protein localization 28.5714 5 no
GO:0002429 immune response-activating cell surface receptor signaling pathway 28.5714 4 yes
GO:0002768 immune response-regulating cell surface receptor signaling pathway 28.5714 4 yes
GO:0050778 positive regulation of immune response 28.5714 5 yes
GO:1901565 organonitrogen compound catabolic process 28.5714 4 yes
GO:1903522 regulation of blood circulation 25.7143 5 yes
GO:0001934 positive regulation of protein phosphorylation 25.7143 8 no
GO:0007169 cell surface receptor protein tyrosine kinase signaling pathway 25.7143 5 no
GO:0008284 positive regulation of cell population proliferation 25.7143 5 no
GO:0010562 positive regulation of phosphorus metabolic process 25.7143 6 no
GO:0018193 peptidyl-amino acid modification 25.7143 5 no
GO:0042327 positive regulation of phosphorylation 25.7143 8 no
GO:0045937 positive regulation of phosphate metabolic process 25.7143 7 no
GO:0051336 regulation of hydrolase activity 25.7143 4 no
GO:0045935 positive regulation of nucleobase-containing compound metabolic process 25.7143 5 no
GO:0015031 protein transport 25.7143 4 no
GO:0070201 regulation of establishment of protein localization 25.7143 6 no
GO:0031400 negative regulation of protein modification process 25.7143 7 yes
GO:0050851 antigen receptor-mediated signaling pathway 25.7143 5 yes
GO:0071417 cellular response to organonitrogen compound 25.7143 4 yes
GO:1901699 cellular response to nitrogen compound 25.7143 4 yes
GO:0010975 regulation of neuron projection development 22.8571 7 no
GO:0033993 response to lipid 22.8571 4 no
GO:0043085 positive regulation of catalytic activity 22.8571 4 no
GO:0045597 positive regulation of cell differentiation 22.8571 5 no
GO:0007010 cytoskeleton organization 22.8571 5 no
GO:0022603 regulation of anatomical structure morphogenesis 22.8571 4 no
GO:0030100 regulation of endocytosis 22.8571 5 no
GO:0043066 negative regulation of apoptotic process 22.8571 6 no
GO:0043069 negative regulation of programmed cell death 22.8571 5 no
GO:0045893 positive regulation of DNA-templated transcription 22.8571 8 no
GO:0045944 positive regulation of transcription by RNA polymerase II 22.8571 9 no
GO:0050890 cognition 22.8571 4 no
GO:0051254 positive regulation of RNA metabolic process 22.8571 6 no
GO:0051493 regulation of cytoskeleton organization 22.8571 6 no
GO:1902680 positive regulation of RNA biosynthetic process 22.8571 7 no
GO:1903829 positive regulation of protein localization 22.8571 5 no
GO:1904951 positive regulation of establishment of protein localization 22.8571 6 no
GO:0098657 import into cell 22.8571 4 no
GO:0001819 positive regulation of cytokine production 22.8571 5 yes
GO:0008016 regulation of heart contraction 22.8571 6 yes
GO:0010563 negative regulation of phosphorus metabolic process 22.8571 6 yes
GO:0014070 response to organic cyclic compound 22.8571 4 yes
GO:0032970 regulation of actin filament-based process 22.8571 4 yes
GO:0045936 negative regulation of phosphate metabolic process 22.8571 7 yes
GO:0050852 T cell receptor signaling pathway 20.0000 6 yes
GO:0001933 negative regulation of protein phosphorylation 17.1429 8 yes
GO:0042326 negative regulation of phosphorylation 17.1429 8 yes
GO:0071407 cellular response to organic cyclic compound 17.1429 5 yes
GO:0090257 regulation of muscle system process 17.1429 5 yes
GO:0006937 regulation of muscle contraction 14.2857 6 yes
GO:0006942 regulation of striated muscle contraction 14.2857 7 yes
GO:0032649 regulation of type II interferon production 14.2857 5 yes
GO:0032729 positive regulation of type II interferon production 14.2857 6 yes
GO:0051279 regulation of release of sequestered calcium ion into cytosol 14.2857 5 yes
GO:0051282 regulation of sequestering of calcium ion 14.2857 4 yes
GO:0055117 regulation of cardiac muscle contraction 14.2857 7 yes
GO:1901136 carbohydrate derivative catabolic process 14.2857 4 yes
GO:1903115 regulation of actin filament-based movement 11.4286 5 yes
GO:0002064 epithelial cell development 11.4286 4 yes
GO:0006163 purine nucleotide metabolic process 11.4286 5 yes
GO:0006195 purine nucleotide catabolic process 11.4286 6 yes
GO:0006753 nucleoside phosphate metabolic process 11.4286 4 yes
GO:0009117 nucleotide metabolic process 11.4286 5 yes
GO:0009150 purine ribonucleotide metabolic process 11.4286 6 yes
GO:0009154 purine ribonucleotide catabolic process 11.4286 6 yes
GO:0009166 nucleotide catabolic process 11.4286 6 yes
GO:0009259 ribonucleotide metabolic process 11.4286 5 yes
GO:0009261 ribonucleotide catabolic process 11.4286 5 yes
GO:0019693 ribose phosphate metabolic process 11.4286 4 yes
GO:0032663 regulation of interleukin-2 production 11.4286 5 yes
GO:0032743 positive regulation of interleukin-2 production 11.4286 6 yes
GO:0034655 nucleobase-containing compound catabolic process 11.4286 4 yes
GO:0046434 organophosphate catabolic process 11.4286 4 yes
GO:0071872 cellular response to epinephrine stimulus 11.4286 4 yes
GO:0072521 purine-containing compound metabolic process 11.4286 4 yes
GO:0072523 purine-containing compound catabolic process 11.4286 5 yes
GO:0086004 regulation of cardiac muscle cell contraction 11.4286 6 yes
GO:1901292 nucleoside phosphate catabolic process 11.4286 5 yes
GO:1901361 organic cyclic compound catabolic process 11.4286 4 yes
GO:1902514 regulation of calcium ion transmembrane transport via high voltage-gated calcium channel 11.4286 8 yes
GO:1903523 negative regulation of blood circulation 8.5714 5 yes
GO:1903524 positive regulation of blood circulation 8.5714 5 yes
GO:0006198 cAMP catabolic process 8.5714 7 yes
GO:0009187 cyclic nucleotide metabolic process 8.5714 6 yes
GO:0009214 cyclic nucleotide catabolic process 8.5714 7 yes
GO:0010460 positive regulation of heart rate 8.5714 4 yes
GO:0010880 regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum 8.5714 6 yes
GO:0033135 regulation of peptidyl-serine phosphorylation 8.5714 8 yes
GO:0045822 negative regulation of heart contraction 8.5714 6 yes
GO:0045823 positive regulation of heart contraction 8.5714 6 yes
GO:0046058 cAMP metabolic process 8.5714 7 yes
GO:0052652 cyclic purine nucleotide metabolic process 8.5714 6 yes
GO:0071875 adrenergic receptor signaling pathway 8.5714 4 yes
GO:1901077 regulation of relaxation of muscle 8.5714 6 yes
GO:1901078 negative regulation of relaxation of muscle 8.5714 5 yes
GO:1901897 regulation of relaxation of cardiac muscle 8.5714 7 yes
GO:1901898 negative regulation of relaxation of cardiac muscle 8.5714 6 yes
GO:0001885 endothelial cell development 5.7143 5 yes
GO:0010649 regulation of cell communication by electrical coupling 5.7143 5 yes
GO:0014074 response to purine-containing compound 5.7143 5 yes
GO:0019933 cAMP-mediated signaling 5.7143 5 yes
GO:0032674 regulation of interleukin-5 production 5.7143 5 yes
GO:0032754 positive regulation of interleukin-5 production 5.7143 6 yes
GO:0033137 negative regulation of peptidyl-serine phosphorylation 5.7143 9 yes
GO:0043949 regulation of cAMP-mediated signaling 5.7143 6 yes
GO:0043951 negative regulation of cAMP-mediated signaling 5.7143 7 yes
GO:0046683 response to organophosphorus 5.7143 4 yes
GO:0051591 response to cAMP 5.7143 4 yes
GO:0061028 establishment of endothelial barrier 5.7143 6 yes
GO:0071320 cellular response to cAMP 5.7143 5 yes
GO:1901844 regulation of cell communication by electrical coupling involved in cardiac conduction 5.7143 6 yes

Disease

No data found.

All partners

Uniprot ID Details Highest evidence Localizing into PSD HPA (protein expression in neurons)
ADRB1_HUMAN [view entry] [view interactions] Low throughput yes yes
ARRB2_HUMAN [view entry] [view interactions] Low throughput yes no
AKA12_HUMAN [view entry] [view interactions] Low throughput yes no
SHAN2_HUMAN [view entry] [view interactions] Low throughput yes yes
RYR1_HUMAN [view interactions] Low throughput no no
MYOME_HUMAN [view interactions] Low throughput no no
RACK1_HUMAN [view interactions] Low throughput no yes
ARRB1_HUMAN [view entry] [view interactions] Low throughput yes yes
AKAP6_HUMAN [view interactions] Low throughput no no
MDM2_HUMAN [view entry] [view interactions] Low throughput yes yes
ITSN1_HUMAN [view entry] [view interactions] Low throughput yes yes
PP2BA_HUMAN [view entry] [view interactions] Low throughput yes yes
PP2BB_HUMAN [view entry] [view interactions] Low throughput yes yes
CANB1_HUMAN [view entry] [view interactions] Low throughput yes yes
LIS1_HUMAN [view entry] [view interactions] Low throughput yes yes
LYN_HUMAN [view entry] [view interactions] Low throughput yes no
SRC_HUMAN [view entry] [view interactions] Low throughput yes no
FYN_HUMAN [view entry] [view interactions] Low throughput yes no
PAQRB_HUMAN [view interactions] Low throughput no no
CUL1_HUMAN [view interactions] Low throughput no yes
FBX2_HUMAN [view entry] [view interactions] Low throughput yes yes
INAR2_HUMAN [view interactions] Low throughput no no
PDE4D_HUMAN [view entry] [view interactions] Low throughput yes yes
GSK3B_HUMAN [view entry] [view interactions] Low throughput yes yes
KC1A_HUMAN [view interactions] Low throughput no yes
KAPCA_HUMAN [view entry] [view interactions] Low throughput yes yes
PIAS4_HUMAN [view interactions] Low throughput no yes
MK01_HUMAN [view entry] [view interactions] Low throughput yes yes
ABL1_HUMAN [view entry] [view interactions] Low throughput yes yes
GRB2_HUMAN [view entry] [view interactions] Low throughput yes yes
SPTN1_HUMAN [view entry] [view interactions] Low throughput yes yes
P85A_HUMAN [view interactions] Low throughput no yes
CF418_HUMAN [view interactions] High throughput no yes
LMX1B_HUMAN [view interactions] High throughput no no
DYR1A_HUMAN [view interactions] High throughput no yes
COIL_HUMAN [view interactions] High throughput no yes
MAGA6_HUMAN [view interactions] High throughput no no
GOG8G_HUMAN [view interactions] High throughput no no
GOG8F_HUMAN [view interactions] High throughput no no
GOG8D_HUMAN [view interactions] High throughput no no
TRAF1_HUMAN [view interactions] High throughput no no
BUB1_HUMAN [view interactions] High throughput no no
PCNA_HUMAN [view interactions] High throughput no no
ZDH17_HUMAN [view entry] [view interactions] High throughput yes yes
TRI23_HUMAN [view interactions] High throughput no yes
CDK2_HUMAN [view interactions] High throughput no yes
NPHP4_HUMAN [view interactions] High throughput no yes
1433Z_HUMAN [view entry] [view interactions] High throughput yes yes
ZBTB1_HUMAN [view interactions] High throughput no no
IP3KB_HUMAN [view interactions] High throughput no no
F262_HUMAN [view interactions] High throughput no no
PTN13_HUMAN [view interactions] High throughput no yes
PDLI5_HUMAN [view entry] [view interactions] High throughput yes no
SMAP1_HUMAN [view interactions] High throughput no yes
CE152_HUMAN [view interactions] High throughput no yes
SMO_HUMAN [view entry] [view interactions] High throughput yes yes
ATOH1_HUMAN [view interactions] High throughput no no
DS13A_HUMAN [view interactions] High throughput no no
PDE4B_HUMAN [view entry] [view interactions] High throughput yes yes
KALRN_HUMAN [view entry] [view interactions] High throughput yes yes
TRI25_HUMAN [view interactions] High throughput no yes
HNRPL_HUMAN [view interactions] High throughput no yes
VIR_HUMAN [view interactions] High throughput no yes
PLK1_HUMAN [view interactions] High throughput no yes
LRC31_HUMAN [view interactions] High throughput no no
KCC2A_HUMAN [view entry] [view interactions] High throughput yes yes
MKRN2_HUMAN [view interactions] High throughput no yes
OXSR1_HUMAN [view interactions] High throughput no yes
ABCF3_HUMAN [view entry] [view interactions] High throughput yes yes
CLPB_HUMAN [view interactions] High throughput no yes
PDXD1_HUMAN [view interactions] High throughput no no
PDE4A_HUMAN [view interactions] High throughput no no
PGAM2_HUMAN [view entry] [view interactions] High throughput yes no
SOX2_HUMAN [view interactions] High throughput no yes
A4_HUMAN [view entry] [view interactions] High throughput yes yes
RASH_HUMAN [view entry] [view interactions] High throughput yes no
FBX7_HUMAN [view interactions] High throughput no yes
BIN1_HUMAN [view entry] [view interactions] High throughput yes yes
HDGR2_HUMAN [view interactions] High throughput no yes