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KS6A1_HUMAN

(Microbial infection) Promotes the late transcription and translation of viral lytic genes during Kaposis sarcoma-associated herpesvirus/HHV-8 infection, when constitutively activated. [View more on UniProt]

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No data found.

ELM instance Name Type Start End Partner
ELMI002942 DOC_MAPK_RevD_3 DOC 712 726 -
ELMI002942 DOC_MAPK_RevD_3 DOC 712 726 -

Molecular function

Term Name % Distance from the top of the tree Annotated in KS6A1_HUMAN
GO:0000166 nucleotide binding 40.2174 4 yes
GO:0043168 anion binding 40.2174 4 yes
GO:0017076 purine nucleotide binding 39.1304 5 yes
GO:0032555 purine ribonucleotide binding 35.8696 4 yes
GO:0035639 purine ribonucleoside triphosphate binding 35.8696 4 yes
GO:0030554 adenyl nucleotide binding 32.6087 6 yes
GO:0005524 ATP binding 29.3478 5 yes
GO:0032559 adenyl ribonucleotide binding 29.3478 5 yes
GO:0043169 cation binding 28.2609 4 yes
GO:0046872 metal ion binding 28.2609 5 yes
GO:0016301 kinase activity 18.4783 4 yes
GO:0016773 phosphotransferase activity, alcohol group as acceptor 17.3913 4 yes
GO:0004674 protein serine/threonine kinase activity 15.2174 4 yes
GO:0106310 protein serine kinase activity 13.0435 4 yes
GO:0000287 magnesium ion binding 5.4348 6 yes
GO:0004712 protein serine/threonine/tyrosine kinase activity 5.4348 4 yes
GO:0004711 ribosomal protein S6 kinase activity 4.3478 5 yes
GO:0043027 cysteine-type endopeptidase inhibitor activity involved in apoptotic process 3.2609 4 yes

Biological process

Term Name % Distance from top the of the tree Annotated in KS6A1_HUMAN
GO:0048522 positive regulation of cellular process 60.8696 4 yes
GO:0060255 regulation of macromolecule metabolic process 57.6087 4 yes
GO:0048523 negative regulation of cellular process 56.5217 4 yes
GO:0080090 regulation of primary metabolic process 56.5217 4 yes
GO:0031323 regulation of cellular metabolic process 56.5217 4 yes
GO:0009889 regulation of biosynthetic process 47.8261 4 yes
GO:0010556 regulation of macromolecule biosynthetic process 46.7391 5 yes
GO:0010646 regulation of cell communication 46.7391 4 yes
GO:0031326 regulation of cellular biosynthetic process 46.7391 5 yes
GO:0051128 regulation of cellular component organization 45.6522 4 yes
GO:0051246 regulation of protein metabolic process 45.6522 5 yes
GO:0009893 positive regulation of metabolic process 44.5652 4 yes
GO:0009966 regulation of signal transduction 43.4783 4 yes
GO:0010468 regulation of gene expression 43.4783 6 yes
GO:0010604 positive regulation of macromolecule metabolic process 43.4783 5 yes
GO:0031325 positive regulation of cellular metabolic process 41.3043 5 yes
GO:0051049 regulation of transport 34.7826 4 no
GO:0009892 negative regulation of metabolic process 34.7826 4 yes
GO:0019219 regulation of nucleobase-containing compound metabolic process 34.7826 5 yes
GO:0009891 positive regulation of biosynthetic process 33.6957 5 yes
GO:0010605 negative regulation of macromolecule metabolic process 33.6957 5 yes
GO:0008104 protein localization 32.6087 4 no
GO:0010557 positive regulation of macromolecule biosynthetic process 32.6087 6 yes
GO:0031328 positive regulation of cellular biosynthetic process 32.6087 6 yes
GO:0048584 positive regulation of response to stimulus 31.5217 4 no
GO:1902531 regulation of intracellular signal transduction 30.4348 5 yes
GO:0051247 positive regulation of protein metabolic process 30.4348 6 no
GO:0042981 regulation of apoptotic process 30.4348 5 yes
GO:0043067 regulation of programmed cell death 30.4348 4 yes
GO:0051252 regulation of RNA metabolic process 29.3478 5 yes
GO:0031324 negative regulation of cellular metabolic process 29.3478 5 no
GO:0031399 regulation of protein modification process 29.3478 6 no
GO:0023056 positive regulation of signaling 29.3478 4 no
GO:0006796 phosphate-containing compound metabolic process 29.3478 4 yes
GO:2001141 regulation of RNA biosynthetic process 28.2609 6 yes
GO:0010647 positive regulation of cell communication 28.2609 5 no
GO:0006355 regulation of DNA-templated transcription 28.2609 7 yes
GO:0045935 positive regulation of nucleobase-containing compound metabolic process 27.1739 5 yes
GO:0051094 positive regulation of developmental process 27.1739 4 yes
GO:0019220 regulation of phosphate metabolic process 27.1739 6 no
GO:0051174 regulation of phosphorus metabolic process 27.1739 5 no
GO:0009967 positive regulation of signal transduction 27.1739 5 no
GO:0006996 organelle organization 26.0870 4 no
GO:0043085 positive regulation of catalytic activity 26.0870 4 no
GO:0051130 positive regulation of cellular component organization 26.0870 5 no
GO:0060341 regulation of cellular localization 26.0870 4 no
GO:0042127 regulation of cell population proliferation 26.0870 4 no
GO:0051240 positive regulation of multicellular organismal process 25.0000 4 yes
GO:0009890 negative regulation of biosynthetic process 25.0000 5 no
GO:0009894 regulation of catabolic process 25.0000 4 no
GO:0010558 negative regulation of macromolecule biosynthetic process 25.0000 6 no
GO:0031327 negative regulation of cellular biosynthetic process 25.0000 6 no
GO:0043933 protein-containing complex organization 25.0000 4 no
GO:0071310 cellular response to organic substance 25.0000 4 no
GO:0022607 cellular component assembly 25.0000 4 no
GO:0036211 protein modification process 25.0000 4 yes
GO:0043412 macromolecule modification 25.0000 4 yes
GO:0045595 regulation of cell differentiation 25.0000 4 yes
GO:0048585 negative regulation of response to stimulus 23.9130 4 yes
GO:0051248 negative regulation of protein metabolic process 23.9130 6 yes
GO:0010648 negative regulation of cell communication 23.9130 5 yes
GO:0023057 negative regulation of signaling 23.9130 4 yes
GO:0032880 regulation of protein localization 22.8261 5 no
GO:0071702 organic substance transport 22.8261 4 no
GO:0009968 negative regulation of signal transduction 22.8261 5 yes
GO:0051336 regulation of hydrolase activity 21.7391 4 yes
GO:0033043 regulation of organelle organization 21.7391 5 no
GO:0141124 intracellular signaling cassette 21.7391 4 no
GO:0043066 negative regulation of apoptotic process 21.7391 6 yes
GO:0043069 negative regulation of programmed cell death 21.7391 5 yes
GO:0080134 regulation of response to stress 20.6522 4 no
GO:0042325 regulation of phosphorylation 20.6522 7 no
GO:0045597 positive regulation of cell differentiation 20.6522 5 yes
GO:0051254 positive regulation of RNA metabolic process 19.5652 6 yes
GO:0016310 phosphorylation 19.5652 5 yes
GO:0030162 regulation of proteolysis 19.5652 6 yes
GO:0006357 regulation of transcription by RNA polymerase II 18.4783 8 yes
GO:1902680 positive regulation of RNA biosynthetic process 17.3913 7 yes
GO:0045893 positive regulation of DNA-templated transcription 17.3913 8 yes
GO:0006468 protein phosphorylation 16.3043 5 yes
GO:1902532 negative regulation of intracellular signal transduction 15.2174 6 yes
GO:0006417 regulation of translation 14.1304 6 yes
GO:0010608 post-transcriptional regulation of gene expression 14.1304 7 yes
GO:0045944 positive regulation of transcription by RNA polymerase II 11.9565 9 yes
GO:0052547 regulation of peptidase activity 11.9565 5 yes
GO:0052548 regulation of endopeptidase activity 11.9565 6 yes
GO:0001558 regulation of cell growth 11.9565 4 yes
GO:0043086 negative regulation of catalytic activity 11.9565 4 yes
GO:0045927 positive regulation of growth 10.8696 4 yes
GO:0050865 regulation of cell activation 9.7826 4 yes
GO:0098916 anterograde trans-synaptic signaling 9.7826 6 yes
GO:0099536 synaptic signaling 9.7826 4 yes
GO:0099537 trans-synaptic signaling 9.7826 5 yes
GO:2000116 regulation of cysteine-type endopeptidase activity 9.7826 7 yes
GO:0007268 chemical synaptic transmission 9.7826 7 yes
GO:0032006 regulation of TOR signaling 9.7826 6 yes
GO:0050867 positive regulation of cell activation 8.6957 5 yes
GO:0045861 negative regulation of proteolysis 8.6957 7 yes
GO:0051346 negative regulation of hydrolase activity 7.6087 5 yes
GO:0030307 positive regulation of cell growth 7.6087 5 yes
GO:0043281 regulation of cysteine-type endopeptidase activity involved in apoptotic process 7.6087 8 yes
GO:0032007 negative regulation of TOR signaling 6.5217 7 yes
GO:0010466 negative regulation of peptidase activity 5.4348 6 yes
GO:0010951 negative regulation of endopeptidase activity 5.4348 7 yes
GO:2000117 negative regulation of cysteine-type endopeptidase activity 4.3478 8 yes
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process 4.3478 7 yes
GO:0043555 regulation of translation in response to stress 3.2609 7 yes
GO:0072574 hepatocyte proliferation 2.1739 5 yes
GO:0072575 epithelial cell proliferation involved in liver morphogenesis 2.1739 4 yes
GO:2000489 regulation of hepatic stellate cell activation 2.1739 5 yes
GO:2000491 positive regulation of hepatic stellate cell activation 2.1739 6 yes

Disease

No data found.

Uniprot ID Details Highest evidence Localizing into PSD HPA (protein expression in neurons)
MDM2_HUMAN [view entry] [view interactions] Low throughput yes yes
BAD_HUMAN [view entry] [view interactions] Low throughput yes yes
CHSP1_HUMAN [view interactions] Low throughput no yes
PDIP3_HUMAN [view interactions] Low throughput no no
ESR1_HUMAN [view interactions] Low throughput no no
PDCD4_HUMAN [view interactions] Low throughput no yes
RPTOR_HUMAN [view interactions] Low throughput no yes
TAU_HUMAN [view entry] [view interactions] Low throughput yes yes
GSK3B_HUMAN [view entry] [view interactions] Low throughput yes yes
RHG07_HUMAN [view interactions] Low throughput no no
DPTOR_HUMAN [view interactions] Low throughput no no
P53_HUMAN [view interactions] Low throughput no no
ATM_HUMAN [view interactions] Low throughput no yes
STAT3_HUMAN [view entry] [view interactions] Low throughput yes yes
MAGI1_HUMAN [view interactions] Low throughput no no
MK01_HUMAN [view entry] [view interactions] Low throughput yes yes
MK03_HUMAN [view entry] [view interactions] Low throughput yes yes
MTOR_HUMAN [view entry] [view interactions] Low throughput yes yes
1433B_HUMAN [view entry] [view interactions] Low throughput yes yes
SCRIB_HUMAN [view entry] [view interactions] Low throughput yes no
VASP_HUMAN [view entry] [view interactions] Low throughput yes yes
ENAH_HUMAN [view entry] [view interactions] Low throughput yes yes
AKAP8_HUMAN [view interactions] Low throughput no yes
PYGM_HUMAN [view entry] [view interactions] Low throughput yes no
ACTB_HUMAN [view entry] [view interactions] Low throughput yes no
AGO2_HUMAN [view entry] [view interactions] Low throughput yes yes
FXR2_HUMAN [view entry] [view interactions] Low throughput yes yes
CDN1B_HUMAN [view interactions] Low throughput no yes
EF2K_HUMAN [view entry] [view interactions] Low throughput yes no
CREB1_HUMAN [view interactions] Low throughput no yes
IF4B_HUMAN [view entry] [view interactions] Low throughput yes yes
RS6_HUMAN [view entry] [view interactions] Low throughput yes yes
MAD1_HUMAN [view interactions] Low throughput no yes
S100B_HUMAN [view entry] [view interactions] Low throughput yes yes
KS6A1_HUMAN [view entry] [view interactions] Low throughput yes yes
FGFR1_HUMAN [view entry] [view interactions] Low throughput yes yes
MK07_HUMAN [view interactions] Low throughput no yes
ETV1_HUMAN [view interactions] Low throughput no no
TSC1_HUMAN [view entry] [view interactions] Low throughput yes yes
TSC2_HUMAN [view entry] [view interactions] Low throughput yes yes
IKBA_HUMAN [view interactions] Low throughput no yes
TOB1_HUMAN [view interactions] Low throughput no yes
CBP_HUMAN [view interactions] Low throughput no yes
YBOX1_HUMAN [view interactions] Low throughput no yes
UBR5_HUMAN [view interactions] Low throughput no no
1433F_HUMAN [view entry] [view interactions] High throughput yes yes
BCAR1_HUMAN [view interactions] High throughput no yes
1433T_HUMAN [view entry] [view interactions] High throughput yes yes
GOPC_HUMAN [view entry] [view interactions] High throughput yes yes
SNTB2_HUMAN [view entry] [view interactions] High throughput yes no
HMGB2_HUMAN [view interactions] High throughput no yes
PICAL_HUMAN [view entry] [view interactions] High throughput yes yes
1433G_HUMAN [view entry] [view interactions] High throughput yes yes
GBP6_HUMAN [view interactions] High throughput no yes
WASC4_HUMAN [view interactions] High throughput no yes
CTBP1_HUMAN [view entry] [view interactions] High throughput yes yes
EGFR_HUMAN [view interactions] High throughput no no
SPPL3_HUMAN [view interactions] High throughput no no
KS6B1_HUMAN [view entry] [view interactions] High throughput yes yes
KS6B2_HUMAN [view interactions] High throughput no yes
AKT1_HUMAN [view entry] [view interactions] High throughput yes yes
AKT2_HUMAN [view entry] [view interactions] High throughput yes yes
KS6A5_HUMAN [view entry] [view interactions] High throughput yes yes
SGK2_HUMAN [view interactions] High throughput no yes
AKT3_HUMAN [view interactions] High throughput no yes
UBE2T_HUMAN [view interactions] High throughput no no
ARHGC_HUMAN [view interactions] High throughput no yes
MAST2_HUMAN [view interactions] High throughput no yes
PDZD7_HUMAN [view interactions] High throughput no no
GRD2I_HUMAN [view entry] [view interactions] High throughput yes no
MAST1_HUMAN [view interactions] High throughput no yes
PAR3L_HUMAN [view interactions] High throughput no yes
PZRN4_HUMAN [view interactions] High throughput no no
HTRA1_HUMAN [view interactions] High throughput no yes
MAGI2_HUMAN [view entry] [view interactions] High throughput yes yes
SNTG2_HUMAN [view interactions] High throughput no no
GORS2_HUMAN [view interactions] High throughput no yes
NHRF3_HUMAN [view interactions] High throughput no no
SNTA1_HUMAN [view entry] [view interactions] High throughput yes no
GRASP_HUMAN [view entry] [view interactions] High throughput yes yes
MAGI3_HUMAN [view interactions] High throughput no no
ARHGB_HUMAN [view interactions] High throughput no yes
SHAN3_HUMAN [view entry] [view interactions] High throughput yes no
SYJ2B_HUMAN [view interactions] High throughput no no
PZRN3_HUMAN [view interactions] High throughput no yes
WHRN_HUMAN [view interactions] High throughput no no
SNTB1_HUMAN [view entry] [view interactions] High throughput yes yes
NHRF2_HUMAN [view interactions] High throughput no no
SHRM2_HUMAN [view interactions] High throughput no yes
LIMK2_HUMAN [view interactions] High throughput no yes
GORS1_HUMAN [view interactions] High throughput no yes
LNX1_HUMAN [view entry] [view interactions] High throughput yes yes
DLG4_HUMAN [view entry] [view interactions] High throughput yes yes
ERBIN_HUMAN [view entry] [view interactions] High throughput yes yes
NEB1_HUMAN [view entry] [view interactions] High throughput yes yes
SNX27_HUMAN [view entry] [view interactions] High throughput yes no
IF4H_HUMAN [view interactions] High throughput no yes
PSF2_HUMAN [view interactions] High throughput no no
CAN1_HUMAN [view entry] [view interactions] High throughput yes yes
CASP7_HUMAN [view interactions] High throughput no no
CNDP2_HUMAN [view entry] [view interactions] High throughput yes yes
EF2_HUMAN [view entry] [view interactions] High throughput yes yes
GNS_HUMAN [view interactions] High throughput no yes
GSHR_HUMAN [view interactions] High throughput no yes
KYNU_HUMAN [view interactions] High throughput no no
LDHA_HUMAN [view entry] [view interactions] High throughput yes yes
LDHB_HUMAN [view entry] [view interactions] High throughput yes yes
MVD1_HUMAN [view interactions] High throughput no yes
PSA_HUMAN [view entry] [view interactions] High throughput yes yes
PEPD_HUMAN [view interactions] High throughput no yes
6PGD_HUMAN [view entry] [view interactions] High throughput yes no
ILKAP_HUMAN [view interactions] High throughput no yes
GMFB_HUMAN [view interactions] High throughput no yes
GP162_HUMAN [view interactions] High throughput no yes
RASH_HUMAN [view entry] [view interactions] High throughput yes no
RASK_HUMAN [view entry] [view interactions] High throughput yes no
RASN_HUMAN [view entry] [view interactions] High throughput yes no
LTN1_HUMAN [view interactions] High throughput no no
COPG1_HUMAN [view interactions] High throughput no yes
AP2A2_HUMAN [view entry] [view interactions] High throughput yes yes
AP2M1_HUMAN [view entry] [view interactions] High throughput yes yes
TAP1_HUMAN [view interactions] High throughput no yes
ARAF_HUMAN [view interactions] High throughput no yes
DDX50_HUMAN [view interactions] High throughput no yes
DTNA_HUMAN [view entry] [view interactions] High throughput yes yes
FLOT2_HUMAN [view entry] [view interactions] High throughput yes yes
DOCK4_HUMAN [view entry] [view interactions] High throughput yes no
GSH0_HUMAN [view interactions] High throughput no yes
TELO2_HUMAN [view interactions] High throughput no yes
SGMR2_HUMAN [view interactions] High throughput no no
CNDG2_HUMAN [view interactions] High throughput no yes
RAD21_HUMAN [view interactions] High throughput no yes
CDC45_HUMAN [view interactions] High throughput no no
VPS52_HUMAN [view entry] [view interactions] High throughput yes no
INF2_HUMAN [view entry] [view interactions] High throughput yes yes
MP2K7_HUMAN [view entry] [view interactions] High throughput yes yes
PAPD1_HUMAN [view interactions] High throughput no yes
NCLN_HUMAN [view interactions] High throughput no yes
TBB3_HUMAN [view entry] [view interactions] High throughput yes yes
PLCA_HUMAN [view interactions] High throughput no yes
DHX40_HUMAN [view interactions] High throughput no yes
ALG5_HUMAN [view interactions] High throughput no yes
FRS2_HUMAN [view interactions] High throughput no no
LMF2_HUMAN [view interactions] High throughput no yes
MORC3_HUMAN [view interactions] High throughput no yes
PPM1F_HUMAN [view interactions] High throughput no yes
DJC11_HUMAN [view entry] [view interactions] High throughput yes no
MOT1_HUMAN [view interactions] High throughput no no
AP3M1_HUMAN [view entry] [view interactions] High throughput yes yes
ICMT_HUMAN [view interactions] High throughput no no
KAT7_HUMAN [view interactions] High throughput no yes
LAGE3_HUMAN [view interactions] High throughput no yes
ORC5_HUMAN [view interactions] High throughput no yes
AP4B1_HUMAN [view interactions] High throughput no yes
GGA2_HUMAN [view interactions] High throughput no yes
RFA1_HUMAN [view interactions] High throughput no yes
RFA2_HUMAN [view interactions] High throughput no yes
RFA3_HUMAN [view interactions] High throughput no yes
MUC18_HUMAN [view interactions] High throughput no yes
MERL_HUMAN [view interactions] High throughput no yes
ATX3_HUMAN [view interactions] High throughput no yes
TCPD_HUMAN [view entry] [view interactions] High throughput yes yes
COF1_HUMAN [view entry] [view interactions] High throughput yes no
DDAH2_HUMAN [view interactions] High throughput no yes
GBP2_HUMAN [view interactions] High throughput no yes
EM55_HUMAN [view entry] [view interactions] High throughput yes no
PHAX_HUMAN [view interactions] High throughput no yes
PPM1G_HUMAN [view interactions] High throughput no no
SRSF5_HUMAN [view interactions] High throughput no yes
TTK_HUMAN [view interactions] High throughput no yes
TERA_HUMAN [view entry] [view interactions] High throughput yes yes
KS6A2_HUMAN [view interactions] High throughput no yes
USP9X_HUMAN [view entry] [view interactions] High throughput yes yes
H90B2_HUMAN [view interactions] High throughput no no
CDC37_HUMAN [view interactions] High throughput no yes
IDD_HUMAN [view interactions] High throughput no no
CSK2B_HUMAN [view entry] [view interactions] High throughput yes yes
HSP76_HUMAN [view entry] [view interactions] High throughput yes yes
HSP7C_HUMAN [view entry] [view interactions] High throughput yes yes
BIP_HUMAN [view entry] [view interactions] High throughput yes yes
HS90B_HUMAN [view entry] [view interactions] High throughput yes yes
HS90A_HUMAN [view entry] [view interactions] High throughput yes yes
KS6A3_HUMAN [view entry] [view interactions] High throughput yes yes
MK13_HUMAN [view interactions] High throughput no yes
FRK_HUMAN [view interactions] High throughput no no
AT12A_HUMAN [view entry] [view interactions] High throughput yes no
BASP1_HUMAN [view entry] [view interactions] High throughput yes yes
ACACA_HUMAN [view entry] [view interactions] High throughput yes yes
NFIP1_HUMAN [view interactions] High throughput no yes
EIF3B_HUMAN [view interactions] High throughput no yes
PKN2_HUMAN [view interactions] High throughput no yes
TLK2_HUMAN [view interactions] High throughput no no
GRN_HUMAN [view interactions] High throughput no no
GWL_HUMAN [view interactions] High throughput no yes
S10A1_HUMAN [view interactions] High throughput no yes
S10A2_HUMAN [view interactions] High throughput no no
S10A4_HUMAN [view entry] [view interactions] High throughput yes no
S10A6_HUMAN [view interactions] High throughput no yes
S100P_HUMAN [view interactions] High throughput no no
SCLT1_HUMAN [view interactions] High throughput no yes
FGFR2_HUMAN [view interactions] High throughput no yes
FGFR4_HUMAN [view interactions] High throughput no no
CFTR_HUMAN [view interactions] High throughput no no
NMDZ1_HUMAN [view entry] [view interactions] High throughput yes yes
NMDE2_HUMAN [view entry] [view interactions] High throughput yes no
EED_HUMAN [view interactions] High throughput no yes
NTRK1_HUMAN [view interactions] High throughput no yes
MISP_HUMAN [view interactions] High throughput no no
FYN_HUMAN [view entry] [view interactions] High throughput yes no
IIGP5_HUMAN [view interactions] High throughput no no
I17RA_HUMAN [view interactions] High throughput no yes
F136A_HUMAN [view interactions] High throughput no yes
HDAC2_HUMAN [view interactions] High throughput no yes
PTN3_HUMAN [view interactions] High throughput no yes
PKHA4_HUMAN [view interactions] High throughput no yes
HS71B_HUMAN [view entry] [view interactions] High throughput yes yes
HS71A_HUMAN [view entry] [view interactions] High throughput yes yes
INS_HUMAN [view interactions] High throughput no no
ANDR_HUMAN [view interactions] High throughput no no
PAPOG_HUMAN [view interactions] High throughput no yes
AMHR2_HUMAN [view interactions] High throughput no no
RAB4A_HUMAN [view entry] [view interactions] High throughput yes no
PHKG1_HUMAN [view interactions] High throughput no no
RB27A_HUMAN [view interactions] High throughput no yes
CP141_HUMAN [view interactions] High throughput no no
SRC8_HUMAN [view entry] [view interactions] High throughput yes yes
KS6A4_HUMAN [view interactions] High throughput no yes
TRRAP_HUMAN [view interactions] High throughput no no
PRKN_HUMAN [view entry] [view interactions] High throughput yes yes
ZRAN1_HUMAN [view interactions] High throughput no yes
PARPT_HUMAN [view interactions] High throughput no no
TRI67_HUMAN [view interactions] High throughput no no